| Congresso Brasileiro de Microbiologia 2023 | Resumo: 911-1 | ||||
Resumo:Endophytic fungi are a class of organisms that inhabit internal plant tissues, which is considerably significant in facilitating plant development. These microorganisms have fundamental roles in nutrient absorption from the soil, safeguarding against phytopathogens, and regulating plant responses to stressful environments. This study aimed to investigate the endophytic fungal communities within forage plants from the Brazilian Cerrado biome. Forage plant root samples were collected from organic and conventional farms in Entre Rios de Minas – MG and São Vicente de Minas – MG. Subsequently, genetic material was extracted, and the ITS1 to ITS4 region was amplified using PCR. The purified PCR products were submitted to sequencing on the Illumina MiSeq platform. Mothur software was utilized for OTU designation, while R and RStudio were employed for statistical analyses. A total of 5246 OTUs were identified, with prominent fungal genera including Phoma, Epicoccum, Mortierella, Fusarium, and Lachnum. Based on the alpha diversity parameters, a greater diversity of endophytic fungi was observed in the farms with organic cultivation, as indicated by the number of observed OTUs and the Shannon index (p > 0.05). Notably, beta diversity analysis, through PERMANOVA, revealed discernible differences in fungal communities between the two municipalities (p > 0.001, stress = 0.22), signifying the influence of geographic distribution on the communities’ structure. Furthermore, although statistical differences were observed among different plant groupings (p = 0.001, stress = 0.2), visual distinctions between these groups were not apparent. The core endophyte community exhibited variation across different samples and groupings, but the genera Phoma, Epicoccum, and Mortierella were present in at least 80% of the samples. Based on the data analyzed, we were able to have some insights into the impact of geographic distribution and plant groupings on the endophytic fungal community. Additionally, an investigation of organic cultivation practices revealed a greater diversity. Nevertheless, further in-depth analyses are warranted to elucidate the dynamics of these communities. Palavras-chave: endophytes, plant microbiome, amplicon sequencing Agência de fomento:CAPES, IABS | |||||